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The skin sample was extracted with 1 ml methanol by the same Liquid chromatography-tandem mass spectrometry (UFLC-MS/MS). Ivy extract were diluted 100-fold with methanol/water (50:50) mixture containing 1 mM ammonium acetate followed by ultra-fast Of the vines was prepared by mixing small sections with 5 mL methanol for 4 hours at room temperature. To obtain a comparison standard, poison ivy vines were procured from a single tree in St.
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The scraped material was submitted for chemical analysis by ultra-fast liquid chromatography-tandem mass spectrometry. With a Gillette ® blade easily removed the pigment without bleeding, confirming the superficial location of the pigment-within the stratumĬorneum. Examination was consistent with “black-spot” poison ivy (Figures 1 and 2). The same area that had arisen 5 days prior. Contact, non-polarized image of black-spot urushiolĪ 78-year-old female sought evaluation of pruritic vesicular lesions on the right hand and a 6mm irregular “black spot” in Clinical black-spot urushiol imageįigure 2. List of diagnoses that show a specific dermoscopic pattern.įigure 1. Black-spot poison ivy may be added to the Thisįinding confirmed the presence of multiple urushiol congeners in the skin sample. A skin sample was obtained andĬompared against a poison ivy standard using ultra-fast liquid chromatography-tandem mass spectrometry (UFLC-MS/MS). Dermoscopy revealed a jagged, centrally homogeneous, dark brown lesion with a red rim. The presentation of a black spot on the skin in a clinical context suggesting poison ivy is indicative of black-spot poison A 78-year-old sought evaluation of a black spot present on her right hand amid pruritic vesicles. Missouri University of Science and Technology, Department of Chemistry, Rolla, Missouri Abstractīlack-spot poison ivy is an uncommon presentation of poison ivy ( Toxicodendron) allergic contact dermatitis. Stoecker & Associates, LLC, Rolla, MissouriĢ. Ryan K Rader 1 BS, Ruipu Mu 2 BS, Honglan Shi 2 PhD, William V Stoecker 1 MS MD, Kristen A Hinton 1 BS Dermatology Online Journal 18 (10): 8 1.